Cell proliferation in the murine mammary gland

To investigate proliferation, virgin mice aged 4 weeks (n = 10) were injected with 25mg/kg EdU with a 4 h chase. All mice were estrous matched and examined when in diestrus; vaginal lavages were taken one week prior to sacrifice to ensure cycling. Estrous stage was assessed by relative proportion of nucleated epithelial cells, cornified epithelial cells and leukocytes.

EdU K8 aSMA DAPI

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