Cell proliferation in the murine mammary gland

To investigate proliferation, virgin mice aged 4 weeks (n = 10) were injected with 25mg/kg EdU with a 4 h chase. All mice were estrous matched and examined when in diestrus; vaginal lavages were taken one week prior to sacrifice to ensure cycling. Estrous stage was assessed by relative proportion of nucleated epithelial cells, cornified epithelial cells and leukocytes.


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